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如何回复SCI投稿审稿人意见

时间:2009-12-01  来源:dxy  作者:chenchenx

1.所有问题必须逐条回答。sJx医家园
2.尽量满足意见中需要补充的实验。sJx医家园
3.满足不了的也不要回避,说明不能做的合理理由。sJx医家园
4.审稿人推荐的文献一定要引用,并讨论透彻。sJx医家园
以下是本人对审稿人意见的回复一例,仅供参考。 sJx医家园
Major comments: sJx医家园
1. The authors need to strengthen their results by including MMPsJx医家园
secretion, and tran-matrigel migration by a positive controlsJx医家园
progenitor cell population i.e. enriched human CD34 cellssJx医家园
obtained from mobilized PBL, since this is a more clinicallysJx医家园
relevant source of CD34 cells which has also been shown tosJx医家园
secrete both MMP-9 and MMP-2 (ref. 11). CD34 enriched cellssJx医家园
from steady state peripheral blood which also secrete MMPs aresJx医家园
also of interest. sJx医家园
2. In fig 1C please specify which cell line representssJx医家园
MMP-negative cells. This needs to be clarified, as well as asJx医家园
better explanation of the method of the protocol. sJx医家园
3. The ELISA results are represented as "fold increase" comparedsJx医家园
to control. Instead, we suggest that standards should be used andsJx医家园
results should be presented as absolute concentrations and onlysJx医家园
then can these results be compared to those of the zymography. sJx医家园
4. When discussing the results, the authors should distinguishsJx医家园
clearly between spontaneous migration vs chemotactic migration.sJx医家园
Furthermore, the high spontaneous migration obtained with cordsJx医家园
blood CD34 cells should be compared to mobilized PBL CD34 sJx医家园
enriched cells and discussed. sJx医家园
5. The authors claim that the clonogenic assay was performed tosJx医家园
determine the optimum concentration for inhibition of MMPsJx医家园
activity by phenanthroline and anti MMP-9 mAb, however theysJx医家园
should clarify that this assay can only determine the toxicity ofsJx医家园
the inhibitors and not their optimal inhibitory concentrations.sJx医家园

Minor comments: sJx医家园
1. There are many spelling and syntax errors, especially in thesJx医家园
results and discussion, which need correction. sJx医家园
a. Of special importance, is the percent inhibition of migration,sJx医家园
which is described as percent of migration. i.e. pg 7:"MigrationsJx医家园
of CB CD34 was reduced to 73.3%?" Instead should readsJx医家园
"Migration of CB CD34 was reduced by 73.3%?" sJx医家园
b. The degree symbol needs to be added to the numbers insJx医家园
Materials and methods. sJx医家园
2. It would be preferable to combine figure 1A and B, in order tosJx医家园
confirm the reliability of fig. 1B by a positive controlsJx医家园
(HT1080).sJx医家园

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